More info
Principle
Indirect ELISA, Antigen coated plate
Size
96T
Specificity 99%
Sensitivity 99%
Assay time
~ 75 min
Shelf Life
12 Months from the Manufacturing Date
Storage
Store at 2 - 8ºC.
Sample volume
10 µL Serum or Plasma
Detection Method
Chromogenic
Kit Components
1. 20 x wash buffer: 1vial
2. One 96-well plate precoated with recombinant antigen
3. Assa diluent buffer: 1 vial
4. HRP conjugated: 1 vial
5. Positive Control: 1 vial
6. Negative Control:1vial
7. Calibrator: 1 vial
8. TMB substrate: 1 vial
9. TMB stop solution: 12 ml.
Background
Smith (Sm) antigen is a non-histone nuclear protein composed of several polypeptides of differing molecular weights. Anti-Sm autoantibodies were described originally as precipitating autoantibodies in sera of patients with Systemic Lupus Erythematosus. Approximately 99% of individuals with anti-Sm antibodies have the disease, but only 20% of people with SLE have the antibodies. Sm antibodies are associated with central nervous system involvement, kidney disease, lung fibrosis and pericarditis in SLE, but they are not associated with disease activity. Molecular and epidemiological studies suggest that anti-Sm antibodies may be induced by molecular mimicry because the protein shows some similarity to Epstein-Barr virus proteins.