More info
Principle
Indirect ELSIA, Antigen coated plate
Detection Range
Qualitative Positive; Negative and Cut off
Size
96T
Specificity 98%
Sensitivity 98%
Assay time
~ 75 min
Shelf Life
14 Months from the Manufacturing Date
Storage
Store at 2 - 8ºC.
Sample volume
10 µL Serum or Plasma
Detection Method
Chromogenic
Kit Components
1. 20 x wash buffer: 1vial
2. One 96-well plate precoated with recombinant antigen
3. Assa diluent buffer: 1 vial
4. HRP conjugated: 1 vial
5. Positive Control: 1 vial
6. Negative Control:1vial
7. Calibrator: 1 vial
8. TMB substrate: 1 vial
9. TMB stop solution: 12 ml.
Background
Epstein - Barr virus (EBV) is a ubiquitous human virus, which causes infectious mononucleosis (IM), a self-limiting lymphoproliferative disease. The Epstein-Barr virus viral-capsid antigen (EBV-VCA) is the viral protein that forms the viral capsid of the Epstein-Barr virus. It is the antigen targeted by anti-VCA antibodies. Such antibodies can be used in serology to diagnose infectious mononucleosis. IgG antibodies to viral capsid antigen peak early in an infection and usually persist for life as an indication that a person has been exposed to a pathogen. A rising or high IgG antibody to the viral capsid antigen and negative antibody to EBNA after at least 4 weeks of illness is also strongly suggestive of primary infection.