More info
Principle
Indirect ELSIA, Antigen coated plate
Detection Range
Qualitative Positive; Negative and Cut off
Size
96T
Specificity 93%
Sensitivity 90%
Assay time
~ 100 min
Shelf Life
12~18 Months from the Manufacturing Date
Storage
Store at 2 - 8ºC.
Sample volume
10 µL Serum or Plasma
Detection Method
Chromogenic
Kit Components
1. 20 x wash buffer: 1vial
2. One 96-well plate precoated with recombinant antigen
3. Assa diluent buffer: 1 vial
4. HRP conjugated: 1 vial
5. Positive Control: 1 vial
6. Negative Control:1vial
7. Calibrator: 1 vial
8. TMB substrate: 1 vial
9. TMB stop solution: 12 ml.
Background
Epstein - Barr virus (EBV) is a ubiquitous human virus which causes infectious mononucleosis (IM), a self limiting lymphoproliferative disease. The Epstein-Barr virus viral-capsid antigen (EBV-VCA) is the viral protein that forms the viral capsid of the Epstein-Barr virus. It is the antigen targeted by anti-VCA antibodies. Such antibodies can be used in serology to diagnose infectious mononucleosis. IgM antibodies to a protein on the viral coat (viral capsid antigen) usually appear early in an infection and then disappear they are often a sign of a recent infection or reactivation. A first time EBV infection is indicated if IgM antibody to the viral capsid antigen is present and antibody to EBV nuclear antigen, or EBNA, is absent.