More info
Principle
Indirect ELSIA, Antigen coated plate
Detection Range
Qualitative Positive; Negative and Cut off
Size
96T
Specificity 99%
Sensitivity 99%
Assay time
~ 100 min
Shelf Life
12~18 Months from the Manufacturing Date
Storage
Store at 2 - 8ºC.
Sample volume
10 µL Serum or Plasma
Detection Method
Chromogenic
Kit Components
1. 20 x Wash Buffer: 1vial
2. One 96-well plate precoated with recombinant antigen
3. Assa diluent buffer: 1 vial
4. HRP conjugated: 1 vial
5. Positive Control: 1 vial
6. Negative Control:1vial
7. Calibrator: 1 vial
8. TMB substrate: 1 vial
9. TMB stop solution: 12 ml.
Background
Epstein-Barr virus (EBV), a member of the herpesvirus group, is the etiologic agent of infectious mononucleosis. The Epstein-Barr virus (EBV) early antigen (EA) complex consists of multiple proteins with relevance for diagnosis of acute, chronic and malignant EBV related diseases, including nasopharyngeal carcinoma (NPC). The presence of antibody to the early antigen (EA) of Epstein-Barr virus (EBV) indicates that EBV is actively replicating. Generally, this antibody can only be detected during active EBV infection, such as in patients with infectious mononucleosis. Clinical studies have indicated that patients who have chronic active or reactivated EBV infection commonly have elevated levels of IgG-class antibodies to the EA of EBV.