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Human Leptin ELISA Kit

NR-R10001

$969.60

Data sheet

Assay Range 0 - 100 ng/ml

More info

Sensitivity
0.3 ng/ml
 
Size
96T
 
Storage
Store at 2 - 8ºC. 
 
Assay Principle
Sandwich ELISA
 
Sample volume
15 µL, dilution factor varies on samples.
 
Detection Method
Chromogenic
   
Kit Components
 
 
 
1. Human Leptin standard set: 6 vials
 
 
2. One 96-well plate precoated with anti-human leptin Ab
 
 
3. Assay diluent buffer: 12 mL x 1
 
 
4. Biotinylated leptin detection antibody Ab: 1 vial (11ml)
 
 
5. Strepavidin-HRP solution : 1 vial (11 ml)
 
 
6. 20 x Wash Solution: 25 ml x 1.
 
 
7. TMB substrate: 12 ml.
 
 
8. TMB stop solution: 12 ml.
 
Background

 

Leptin is a secreted hormone made by fat cells, which regulates the amount of fat stored in the body. It may function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Leptin does this by adjusting both the sensation of hunger, and adjusting energy expenditures. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells which have leptin receptors.

standard curve

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