| Assay time |
Extraction & derivatization (2h30), ELISA overnight |
| Assay Range |
0 / 25 - 2 500 ng/ml, 0 / 75 – 7 500 ng/ml |
| Sensitivity |
Depending on sample matrix and volume |
| Assay principle |
Enzyme Immunoassay for the quantitative determination of Gamma-aminobutyric acid (GABA) in human plasma, serum, urine and various biological samples.
After extraction and derivatization Gamma-aminobutyric acid (GABA) is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized analyte concentrations of the standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.
Quantification of unknown samples is achieved by comparing their absorbance with a standard curve
prepared with known standards.
|
| Specific Activity |
Reacts with all species |
| Sample |
100 - 300 µl, all biological samples |
