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Alkaline Phosphatase Bovine Intestinal ( Alkaline Phosphatase Bovine )
DescriptionThe Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to
Data sheet
| Formulation | 50% glycerol, 5mM MgCl2, 0.1mM ZnCl2 and 5mM TRIS, pH 7.0. |
| Purity | 95% pure by Gel Filtration. |
| Description | The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity. |
| Expression host | Calf Intestine. |
| Synonyms | EC 3.1.3.1, IAP, AP, ALPI, Intestinal-type alkaline phosphatase, Intestinal alkaline phosphatase. |
| Reagent Appearance | Sterile Filtered clear colorless solution. |
| Stability | AP should be stored at 4°C. Please Do-Not freeze. |
| Biological Activity | >1000 U/mg (pH 9.6, 25°C and 0.025M glycin, 10% glycerol as buffer. |