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Cardiac Actin ( c-Actin )
DescriptionUltra pure Cardiac Actin having a Molecular mass of 43,000 dalton.SourceBovine Heart.ApplicationsProtein standard in 1D and 2D SDS gelelectrophoresisImmunoassays Immunization.Physical AppearanceSterile Filtered White lyophilized
Data sheet
| Formulation | The protein was lyophilized from a 1mg/ml solution containing 10mM Tris-acetate buffer pH 8.0, 0.2mM CaCl2, 0.2mM ATP, 1mM DTT and 0.5% SDS. |
| Solubility | It is recommended to reconstitute the lyophilized Cardiac Actin in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Applications | Protein standard in 1D and 2D SDS gelelectrophoresisImmunoassays Immunization. |
| Description | Ultra pure Cardiac Actin having a Molecular mass of 43,000 dalton. |
| Protein Background | Alpha-cardiac actin belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in the cardiac actin have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). |
| Expression host | Bovine Heart. |
| Synonyms | Actin alpha cardiac muscle 1, Alpha-cardiac actin, ACTC1, ACTC. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized Cardiac-Actin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Cardiac Actin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |