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Platelet Factor-4 Human Recombinant ( CXCL4 ) ( PF 4 Human )
DescriptionCXCL4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 70 amino acids and having a molecular mass of 7.8 kDa.SourceEscherichia Coli.Physical AppearanceSterile Filtered white lyophilized
Data sheet
| Formulation | The CXCL4 protein was lyophilized in PBS buffer pH-7.4. |
| Solubility | It is recommended to reconstitute the lyophilized CXCL4 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Description | Human PF-4 a 7.8 kDa protein consisting of 70 amino acid residues. |
| Protein Background | Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair. Oncostatin-A is a member of the CXC chemokine family. Human PF4 is used for the proof of heparin-induced thrombocytopenia. Furthermore it is used as an inhibitor in the angiogenesis during tumor therapy. |
| Expression host | Human Platelets. |
| Synonyms | CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298. |
| Reagent Appearance | Sterile Filtered white lyophilized powder. |
| Stability | Human CXCL4 although stable at 25°C 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles. |
| Amino acid sequence | The sequence of the first four N-terminal amino acids was determined and was found to be Glu-Ala-Glu-Glu. |
| Biological Activity | The ED50 of CXCL4 as determined by its ability to inhibit human FGF basic dependent proliferation of NR6R3T3 mouse fibroblasts was found to be 5-15 ug/ml. |