More info
Assay range | 2.5-80 mU |
Sensitivity | 2.5 mU |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | plasma,serum and cell culture supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human VWF standard: 1 vial
2. One 96-well plate coated withHuman VWF Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human VWF Ab (80x) : 100 µL
5. Streptavidin-HRP (100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Von Willebrand factor (VWF) is a 2050 amino acid glycoprotein. Every monomer contains a number of specific domains including the D'/D3 domain, the A1 domain, the A3 domain, the C1 domain and the cysteine knot domain at the C-terminus of the protein. Monomers are subsequently N-glycosylated, arranged into dimers in the endoplasmic reticulum and into multimers in the Golgi apparatus by crosslinking of cysteine residues via disulfide bonds. VWF plays a major role in blood coagulation by binding to factor VIII, collagen, platelet gpIb, plate receptors. Therefore, VWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency. Increased plasma levels in a large number of cardiovascular, neoplastic, and connective tissue diseases have been observed and may contribute to an increased risk of thrombosis.