More info
Assay range | 1.48-120 ng/ml |
Sensitivity | 1.4 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | plasma, milk and cell culture supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human TAT standard: 1 vial
2. One 96-well plate coated withHuman TAT Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human TAT Ab (50x) : 140 µL
5. Streptavidin-HRP (100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Antithrombin III, is a 432 amino acid glycoprotein. It contains three disulfide bonds and a total of four possible glycosylation sites. Antithrombin III plays an important role in inactivating several enzymes of the coagulation system, such as thrombin, factor Xa. Thrombin-antithrombin (TAT) complexes formed following the neutralization of thrombin by antithrombin III (AT III) are a useful marker to predict morphological changes in chronic aortic dissection. High plasma levels of TAT complexes may lead to alter hemostatic activation in argentine hemorrhagic fever, chronic dialysis patients, and toxemia of pregnancy. Low plasma levels of TAT complexes have been observed in type 1 diabetes, neonatal respiratory distress syndrome and primary untreated cancer.