More info
Assay range | 0.031-32µg/ml |
Sensitivity | 0.03 µg/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | plasma, milk, urine, CSF, and cell culture samples. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human Prothrombin standard: 1 vial
2. One 96-well plate coated with Human Prothrombin Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human Prothrombin Ab (50x) : 140 µL
5. Streptavidin-HRP (100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Prothrombin, also known as coagulation factor II, or Pro-Coagulation Factor II, is an inactive precursor of thrombin which is a serine protease encoded by the F2 gene in humans. Prothrombin is proteolytically cleaved into thrombin in the coagulation cascade, thrombin in turn converts soluble fibrinogen into insoluble strands of fibrin, as well as catalyzing many other coagulation-related reactions. In severe Alzheimer's disease, prothrombin was found within the wall and neuropil surrounding microvessels. In addition, elevated plasma levels of prothrombin was observed in sepsis patients and in chronic gastrointestinal disorders.