More info
Assay range | 0.0488-500 ng/ml |
Sensitivity | 0.04 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | Serum, plasma, body fluids, or cell lysates/supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human ApoC-III standard: 1 vial
2. One 96-well plate coated with Human ApoC-III Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human ApoC-III Ab (80x): 100 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Apolipoprotein C-III, also known as apo-CIII, is a small protein encoded by the APOC3 gene in humans. It is a component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. ApoC-III is produced primarily in the liver and to a lesser degree in the intestine. It plays multiple roles in triglyceride homeostasis. It can promote hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion and attenuate hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). It inhibits lipoprotein lipase and hepatic lipase and interferes with binding of lipoproteins to cell surface heparan sulfate proteoglycans and receptors. It is reported that overexpression of the human ApoC-III gene causes hypertriglyceridemia in transgenic mice. Deficiency of ApoC-III prevents hyperlipidemia induced by apoE overexpression.