Data sheet
| Assay Range | 31.2--2000 pg/ml |
More info
Sensitivity
50 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Rat CD121a standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-Rat CD121a Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Rat CD121a Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
IL1RL1 (also called ST2) is a transmembrane protein with a structure similar to IL-1R1. It is a member of the Toll-like receptor superfamily. IL1RL1 is expressed in several cell types including fibroblasts and mast cells. Soluble IL1RL1 also acts as a negative regulator of Th2 cytokine production and high levels have been reported in several disease states and conditions including asthma, sepsis and myocardial infarction. The ligand for ST2 is the cytokine Interleukin-33 (IL-33). Binding of IL-33 to the ST2 receptor, in response to cardiac disease or injury, such as an ischemic event, elicits a cardio-protective effect resulting in preserved cardiac function.
