Data sheet
| Assay Range | 4.7 - 300 pg/ml |
More info
Sensitivity
4 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CD87 standard:1 ng/vial - 2.
2. One 96-well plate precoated with anti-human CD87 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CD87 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
The Urokinase receptor, also known as uPA receptor or uPAR or CD87 (Cluster of Differentiation 87), is multidomainglycoprotein tethered to the cell membrane with a glycosylphospho -tidylinositol (GPI) anchor. uPAR was originally identified as a saturable binding site for urokinase on the cell surface. uPAR is expressed in neurons of the rolandic area of the brain (at protein level). uPAR binds urokinase and thus restricts plasminogen activation to the immediate vicinity of the cell membrane. It is an important player in the regulation of this process. uPAR has also been involved in various other non-proteolytical processes related to cancer, such as cell migration, cell cycleregulation, and cell adhesion.
