Mouse CSF1R/M-CSFR ELISA Kit

Kit Components

 1. Recombinant Mouse CSF1R standard: 2 vials

 2. One 96-well plate precoated with anti- Mouse CSF1R Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

Background

M-CSF receptor (macrophage colony-stimulating factor 1 receptor, M-CSFR, CSF-1-R), also known as proto-oncogene c-Fms, or CD115, is a member of the type III subfamily of receptor tyrosine kinases. This protein group, including receptors for PDGF and SCF, is characteristic of five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region. Mouse MCSF receptor is synthesized as a 977 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 492 aa extracellular region containing the ligand binding domain, a 25 aa transmembrane domain and a 441 aa cytoplasmic domain. The ECD of mouse MCSF R shares more than 99% and 60% aa identity with rat and human counterparts, respectively. M-CSF binding initiates M-CSFR homodimerization, phosphorylation and activation of a series of signaling molecules, especially PI3 Kinase, P42/44 ERK and cCbl, that play an important role in cell proliferation, survival, differentiation and cytoskeletal reorganization. M-CSF/M-CSFR signaling is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC. It is reported that M-CSFR and integrin αvβ3 share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis.