More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human TNFSF4 standard: 2 vials.
2. One 96-well plate precoated with anti- Human TNFSF4 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
OX40 Ligand (OX40L), also known as tumor necrosis factor superfamily member 4 (TNFSF4), gp34 or CD252, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Human OX40L is synthesized as a 183 amino acid (aa) polypeptide with an N-terminal cytoplasmic domain and a C-terminal extracellular domain. Natural occurring OX40 Ligand exists as a homotrimer and is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. It shares 46% aa sequence identity with the mouse counterpart. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed primarily on activated CD4+ T cells. OX40L is one of the co-stimulatory molecules in the immune system. It has been proposed that the predominant function of OX40/OX40L interaction is to transmit a late costimulatory signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression. Various single nucleotide polymorphisms (SNPs) of the OX40L gene have been identified. It is reported that some of them are associated with systemic lupus erythematosus.