Dog/Canine Brain Derived Neurotrophic Factor BDNF ELISA Kit

Product name:  Dog/Canine Brain Derived Neurotrophic Factor BDNF ELISA Kit

Catalog No. BG-CAN10341

Size: 96T

Range: 31.2 pg/mL -2000 pg/mL

Sensitivity: Less than 2 pg/mL

Storage: Store at 2-8⁰C.

Expiration: Six months.

Application: cell culture, supernatant, serum, plasma and other suitable sample solution.

Kit Components:

1. Lyophilized Canine BDNF standard: 10 ng/tube-2.

2. One 96-well plate precoated with anti-Canine BDNF antibody.

3. Sample diluent buffer: 12 mL - 1

4. Biotinylated anti-Canine BDNF antibody: 130 µL, dilution 1:100.

5. Antibody diluent buffer: 12 ml.

6. Streptavidin-HRP: 130 µL, dilution 1:100.

7. Streptavidin-HRP diluent buffer: 12 ml.

8. TMB color developing agent: 10 ml.

9. TMB stop solution: 10 ml.

10. 20 - Wash Solution: 25 ml

Background

Brain-derived neurotrophic factor (BDNF) is a member of the "neurotrophin" family of growth factors, which are related to the canonical "Nerve Growth Factor" (NGF). Neurotrophic factors are found in the brain and the periphery. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. In the brain, it is active in the hippocampus, cortex, and basal forebrain-areas vital to learning, memory, and higher thinking. BDNF itself is important for long-term memory. BDNF was the second neurotrophic factor to be characterized after nerve growth factor (NGF).