Data sheet
| Background | Angiopoietin is part of a family of vascular growth factors that play a role in embryonic and postnatal angiogenesis. Angiopoietin signaling most directly corresponds with angiogenesis, the process by which new arteries and veins form from preexisting blood vessels. Angiogenesis proceeds through sprouting, endothelial cell migration, proliferation, and vessel destabilization and stabilization. They are responsible for assembling and disassembling the endothelial lining of blood vessels. Deregulation of angiopoietin and the tyrosine kinase pathway is common in blood-related diseases such as diabetes, malaria, sepsis, and pulmonary hypertension. This is demonstrated by an increased ratio of angiopoietin-2 and angiopoietin-1 in blood serum. To be specific, angiopoietin levels provide an indication for sepsis. Research on angiopoietin-2 has shown that it is involved in the onset of septic shock. The combination of fever and high levels of angiopoietin-2 are correlated with a greater prospect of the development of septic shock. It has also been shown that imbalances between angiopoietin-1 and angiopoietin-2 signaling can act independently of each other. One angiopoietin factor can signal at high levels while the other angiopoieting factor remains at baseline level signaling. |
More info
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Assay Range |
156 - 10000 pg/mL |
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Sensitivity |
10.0 pg/mL |
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Specificity |
No cross-reaction with other related substances detected |
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Size |
96T |
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Storage |
Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
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Assay Principle |
Sandwich ELISA |
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Sample Volume |
100 µL final volume, dilution factor varies on samples |
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Sample Type |
Serum or plasma |
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Detection Method |
Chromogenic |
Kit Components
1. Recombinant Canine Angiopoietin-1 standard: 2 vials
2. One 96-well plate coated with Canine Angiopoietin-1 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 1 vial
5. Streptavidin-HRP: 1 vial
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing reagent: 10 mlx1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Introduction
Angiopoietin-1 (Ang-1) is a member of the vascular growth factor family that also includes Ang-2 and Ang-4 in humans. Ang-1 is a secreted glycoprotein characteristic of an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain. Ang-1 exhibits approximately 60% aa sequence identity with Ang-2 and both of them act as a ligand for the receptor tyrosine kinase Tie-2. Integrins may also mediate Ang-1 and Ang-2 activities. Ang-1 is widely expressed in the mouse embryo and in adult human tissues, primarily by endothelial support cells, megakaryocytes and platelets. Ang-1 plays a key role in blood vessel development, remodeling, and maturation. In addition, Ang-1 may help maintain hematopoietic stem cells in a quiescent state within the bone marrow. It also enhances neutrophil and eosinophil adhesion and migration, and regulates blood vessel permeability. In the nervous system, Ang-1 may support neuronal growth, survival, and dendritic organization.