| Clone |
P10A7AT. |
| Applications |
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:100. |
| Protein Background |
Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells.Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer. |
| Synonyms |
TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2. |
| Reagent Appearance |
Sterile filtered colorless solution. |
| Type |
Mouse Anti Human Monoclonal. |
| Immunogen |
Anti-human TNF-a mAb, clone P10A7AT, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TNF-a protein 57-233 amino acids purified from E. coli. |
| Ig Subclass |
Mouse IgG2b heavy chain and k light chain. |
| Purification Method |
TNF-a antibody was purified by protein-A affinity chromatography. |
| Protein formulation |
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide. |
| Storage Procedures |
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. |
| Stability / Shelf Life |
12 months at -20°C. 1 month at 4°C. |
