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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| TNF receptor-associated factor 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human TRAF2(305-325aa RQHRLDQDKIEALSSKVQQLE), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TRAF2 |
Protein | TNF receptor-associated factor 2 |
Uniprot ID | Q12933 |
Function | Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'- linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin- protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the TNF receptor-associated factor family. A subfamily. |
Aliases | E3 ubiquitin-protein ligase TRAF2 antibody|MGC: 45012 antibody|OTTHUMP00000022625 antibody|OTTHUMP00000064745 antibody|TNF receptor associated factor 2 antibody|TNF receptor-associated factor 2 antibody|TNF receptor-associated protein antibody|TRAF 2 antibody|TRAF2 antibody|TRAF2_HUMAN antibody|TRAP 3 antibody|TRAP antibody|TRAP3 antibody|Tumor necrosis factor type 2 receptor associated protein 3 antibody|Tumor necrosis factor type 2 receptor-associated protein 3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TRAF2 antibody, ASA-B1901, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human TRAF2, 30.4KD(162aa tag+ Q261-V369)
Lane 1: Recombinant Human TRAF2 Protein 10ng
Lane 2: Recombinant Human TRAF2 Protein 5ng
Lane 3: Recombinant Human TRAF2 Protein 2.5ng
Recombinant Protein Detection Source: E.coli derived -recombinant Human TRAF2, 30.4KD(162aa tag+ Q261-V369)
Lane 1: Recombinant Human TRAF2 Protein 10ng
Lane 2: Recombinant Human TRAF2 Protein 5ng
Lane 3: Recombinant Human TRAF2 Protein 2.5ng
Anti-TRAF2 antibody, ASA-B1901, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
Anti-TRAF2 antibody, ASA-B1901, ICC
ICC: HELA Cell
ICC: HELA Cell