TRAF2  Antibody View larger

TRAF2 Antibody

ASA-B1902

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

TNF receptor-associated factor 2 Polyclonal IgG Rabbit Human IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human TRAF2 (308-339aa RLDQDKIEALSSKVQQLERSIGLKDLAMADLE), different from the related mouse sequence by one amino acid.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

TRAF2

Protein

TNF receptor-associated factor 2

Uniprot ID

Q12933

Function

Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'- linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin- protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.

Tissue Specificity

Sub-cellular localization

Cytoplasm.

Sequence Similarities

Belongs to the TNF receptor-associated factor family. A subfamily.

Aliases

E3 ubiquitin-protein ligase TRAF2 antibody|MGC:45012 antibody| OTTHUMP00000022625 antibody| OTTHUMP00000064745 antibody|TNF receptor associated factor 2 antibody|TNF receptor-associated factor 2 antibody| TNF receptor-associated protein antibody|TRAF 2 antibody|TRAF2 antibody| TRAF2_HUMAN antibody|TRAP 3 antibody|TRAP antibody|TRAP3 antibody|Tumor necrosis factor type 2 receptor associated protein 3 antibody|Tumor necrosis factor type 2 receptor-associated protein 3 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- TRAF2 antibody, ASA-B1902, Western blotting
All lanes: Anti TRAF2 (ASA-B1902) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 56KD
Observed bind size: 56KD
Anti- TRAF2 antibody, ASA-B1902, IHC(P)
IHC(P): Human Tonsil Tissue

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