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Aminopeptidase Aeromonas Recombinant ( Aminopeptidase )
DescriptionThe 29 kDa Aeromonas Aminopeptidase is produced by genetic engineering and can be used for physical & structural investigations, sequence and amino-terminal determinations. This exopeptidase recognizes a specific stop sign at -X- Pro and
Data sheet
| Formulation | Buffered solution containing 10mM Tris-HCl, 100mM NaCl and 5uM ZnSO4, pH 8.0. |
| Purity | Greater than 95.0% as determined by SEC-HPLC. |
| Description | The 29 kDa Aeromonas Aminopeptidase is produced by genetic engineering and can be used for physical & structural investigations, sequence and amino-terminal determinations. This exopeptidase recognizes a specific stop sign at X- Pro and requires a free a-amino group in the L-configuration. It is therefore suitable for the removal of the redundant N-terminal methionine often added to engineered recombinant proteins. |
| Expression host | Aeromonas Proteolytica. |
| Synonyms | Bacterial leucyl aminopeptidase, EC 3.4.11.10. |
| Reagent Appearance | Sterile filtered liquid formulation. |
| Stability | Two years when stored at -20°C, 2 weeks at 4°C. |
| Biological Activity | Recombinant Aeromonas Aminopeptidase was found to have an activity of 120 Units/mg protein. |
| Unit Defenition | One unit of aminopeptidase activity is defined as the amount of enzyme that releases 1 ?mole p-nitroaniline at 25°C in 1 minute. |