Data sheet
| Assay Range | 12.5 - 800 pg/ml |
More info
Sensitivity
10 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse OMD standard: 8 ng/vial - 2.
2. One 96-well plate precoated with anti-Mouse OMD Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse OMD Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Osteomodulin (OMD), also known as Osteoadherin, is a secreted protein which belongs to the small leucine-rich proteoglycan (SLRP) family and Class II subfamily. It is present in the extracellular matrix of bone and other mineralized tissues. In bone matrix, OSAD is localized into cement lines and mineralizing fronts, being absent into the mineralized cartilage that remain at the center of the bone trabeculae. It has a function in binding of osteoblasts via the alpha (V) beta (3)-integrin. It may be implicated in biomineralization processes.
