| Formulation |
PLGF-1 protein was lyophilized from a 0.2um filtered solution in Acetonitrile and TFA. |
| Solubility |
It is recommended to reconstitute the lyophilized PLGF1 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity |
Greater than 97.0% as determined by SDS-PAGE. |
| Description |
PLGF1 Human Recombinant (21-221 a.a.) produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 129 amino acids and having a molecular mass of 29kDa.The PLGF-1 Human Recombinant protein is purified by proprietary chromatographic techniques. |
| Protein Background |
PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It binds to receptor vegfr-1/flt1. |
| Expression host |
Escherichia coli. |
| Synonyms |
PIGF, PGF, PLGF-1, PlGF-2. |
| Reagent Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability |
Lyophilized PLGF-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Amino acid sequence |
AVPPQQWALS AGNGSSEVEV VPFQEVWGRS YCRALERLVD VVSEYPSEVE HMFSPSCVSL LRCTGCCGDE NLHCVPVETA NVTMQLLKIR SGDRPSYVEL TFSQHVRCEC RPLREKMKPE RCGDAVPRR. |
| Biological Activity |
Binds to immobilized rHuVEGFR1 with EC50 less than 6ng/ml. |
