| Reconstitution |
1mg/ml in PBS (after reconstitution). |
| Formulation |
The HSPB2 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 20% glycerol. |
| Solubility |
This antibody was produced in BALB/c mice. |
| Purity |
Greater than 90% as determined by SDS-PAGE. |
| Description |
HSPB2 Human Recombinant produced in E. coli is a single polypeptide chain containing 206 amino acids (1-182) and having a molecular mass of 22.8kDa.HSPB2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
Heat shock protein beta-2 (HSPB2) is a member of the small heat shock protein (HSP20) family. HSPB2 is a regulator of DMPK kinase. |
| Expression host |
E.coli. |
| Synonyms |
Heat shock 27kDa protein 2, DMPK-binding protein, MKBP, HSP27, LOH11CR1K. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSHMSGRSV PHAHPATAEY EFANPSRLGE QRFGEGLLPE EILTPTLYHG YYVRPRAAPA GEGSRAGASE LRLSEGKFQA FLDVSHFTPD EVTVRTVDNL LEVSARHPQR LDRHGFVSRE FCRTYVLPAD VDPWRVRAAL SHDGILNLEA PRGGRHLDTE VNEVYISLLP APPDPEEEEE AAIVEP. |
| Antibody Properties |
Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Titer |
Antibody is shipped lyophilized at ambient temperature. |
| Protein content |
By direct ELISA, 1:20,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). |
| Amino Acid Sequences |
No cross reactivity with murine IL-8 was detected. |
