| Specificity |
reactive with Mouse Lipocalin-2，reactivity with other related proteins not observed |
| Assay Range |
7.81-500 pg/ml |
| Sensitivity |
1.75 pg/ml |
| Size |
96 Tests |
| Storage |
2-8℃ |
| Background |
The lipocalins are a family of proteins which transport small hydrophobic molecules such as steroids, bilins, retinoids, and lipids. They share limited regions of sequence homology and a common tertiary structure architecture.This is an eight stranded antiparallel beta-barrel with a repeated + 1 topology enclosing an internal ligand binding site. |
| Assay principle |
This Mouse Lipocalin-2 ELISA Kit was based on standard sandwich enzyme-linked immunosorbent assay technology. Mouse Lipocalin-2 specific antibodies were precoated onto 96-well plates. The Mouse Lipocalin-2 specific detection antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing the plate. Streptavidin-HRP was added and unbound conjugates were washed away with wash buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow color is proportional to the Mouse Lipocalin-2 amount of sample captured in plate. |
| Sample type |
serum，plasma，cell culture supernatant |
| Assay duration |
4 hours |
| Assay format |
Sandwich |
| Alternative Name of Analyte |
24p3；MSFI；NGAL |
