More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human IL-20 standard: 2 vials
2. One 96-well plate precoated with anti-Human IL-20 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Interleukin-20 (IL-20), also known as cytokine Zcyto10, is a member of the IL-10 family. Human IL-20 is synthesized as a 176 amino acid (aa) precursor consisting of a 24 aa signal peptide and a 152 aa mature fragment. IL-20 is produced by activated keratinocytes and monocytes. Expression of IL-20 is induced by LPS.
IL-20 transduces its signals through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. The first complex is composed of IL-20 Rα and IL-20 Rβ shared with IL-19 and IL-24. The other is IL-22 R and IL-20 Rβ, a common receptor complex for IL-24. IL-20 signaling activates STAT3 pathway and stimulates the induction of pro-inflammatory genes including TNF-γ and MCP-1. It is showed that IL-20 has the ability to regulate skin development. Overexpression of human and mouse IL-20 cause keratinocyte hyperproliferation, abnormal epidermal differentiation, and neonatal lethality. In addition, IL-20 promotes the proliferation of multipotential progenitors in vitro, suggesting it might plays a role in hematopoiesis.