Data sheet
| Assay Range | 15.6 - 1000 pg/ml |
More info
Sensitivity
60 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human GRN standard: 4 ng/vial - 2.
2. One 96-well plate precoated with anti-human GRN Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human GRN Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein, progranulin, which is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Both the peptides and intact granulin protein regulate cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
