Human Matrix Metalloproteinase 7 MMP-7 ELISA Kit

Kit Components

 1. Recombinant Human MMP-7 standard: 2 vials

 2. One 96-well plate precoated with anti- Human MMP-7 Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

Background

Matrix metalloproteinase-7 (MMP-7), also known as matrilysin, is a member of the matrix metalloproteinases (MMPs) family belonging to the zinc and calcium dependent endopeptidase superfamily. Matrix Metalloproteinases (MMPs), also called Matrixins, plays an important role in the degradation of the extracellular matrix which is involved in a series of physiological processes related to embryonic development, morphogenesis, and tissue remodeling. Human MMP-7 is secreted as a small non-glycosylated proenzyme (pro-MMP-7) composed of a propeptide with a cysteine switch motif that is conserved in MMPs and keeps MMP-7 in an inactive state, and a mature polypeptide which consists of a catalytic domain with a zinc-binding motif conserved in metzincins. Activation of the mature MMP-7 is achieved by proteolytic removal of the propeptide or through oxygenation of the cysteine switch motif by hypochlorous acid. MMP-7 is expressed in the epithelial cells of normal and diseased tissues. In normal tissues, MMP-7 is expressed in secretory and ductal epithelium in the endometrium and in various exocrine glands. It is also expressed in a variety of tumors ranging from breast, colon, prostate, stomach, upper aerodigestive tract, lung, and skin. MMP-7 can degrade collagen IV, gelatins, laminin, aggrecan, entactin, elastin, and versican. In addition, it activates urokinase plasminogen activator and pro-MMP-1, -2, and -9, to cleave additional substrates such as osteopontin.