| Formulation |
The NRN1 was lyophilized from a 0.2um filtered concentrated solution in 1PBS, pH 7.4. |
| Solubility |
It is recommended to reconstitute the lyophilized NRN1 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity |
Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Description |
Recombinant Human NRN1 produced in E.coli cells is a non-glycosylated, homodimeric protein containing 2x88 amino acid chains and having a molecular mass of 19.4kDa. The NRN1 is purified by proprietary chromatographic techniques. |
| Protein Background |
Neuritin (NRN1) is a neurotrophic factor, which is expressed in response to induction of neuronal activity by NGF, BDNF, NT3 and other neural stimulators. NRN1 is expressed principally in postmitotic-differentiating neurons of the developing nervous system and in neuronal structures related to synaptic plasticity in the adult nervous system. NRN1 functions as a molecular mediator of neurite outgrowth, neuronal survival, and synaptic maturation. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Neuritin 1, NRN1, NRN, dJ380B8.2, Neuritin. |
| Reagent Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability |
Lyophilized NRN1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NRN1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence |
AGKCDAVFKG FSDCLLKLGD SMANYPQGLD DKTNIKTVCT YWEDFHSCTV TALTDCQEGA KDMWDKLRKE SKNLNIQGSL FELCGSGN. |
| Biological Activity |
The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 25ng/ml, corresponding to a specific activity of > 4.0 104 IU/mg. |
