| Formulation |
NARS is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol. |
| Purity |
Greater than 80.0% as determined by SDS-PAGE. |
| Description |
NARS Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 63,853 Dalton. NARS is expressed with a -6xHis tag at N-terminus and purified by proprietary chromatographic techniques. |
| Protein Background |
Aminoacyl-tRNA synthetases are a class of enzymes which charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase (NARS) is localized to the cytoplasm and is a member of the class II family of tRNA synthetases. The N-terminal domain characterizes the signature sequence for the eukaryotic asparaginyl-tRNA synthetases. |
| Expression host |
Sf9 Insect Cells. |
| Synonyms |
Asparagine--tRNA ligase, cytoplasmic, EC 6.1.1.22, Asparaginyl-tRNA synthetase, AsnRS, NARS, NARS1. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
