| Formulation |
MAGOHB protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer, (pH 8.0), 1mM DTT, 30% glycerol and 0.15M NaCl. |
| Purity |
Greater than 90.0% as determined by SDS-PAGE. |
| Description |
MAGOHB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 171 amino acids (1-148 a.a.) and having a molecular mass of 19.7kDa.MAGOHB is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
Mago-Nashi Homolog B (MAGOHB) is a member of the mago nashi family. MAGOHB involved in mRNA splicing and in the nonsense-mediated decay (NMD) pathway. MAGOHB interacts with RBM8A and is a part of the exon junction complex (EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, UPF3B, UPF2 and ALYREF/THOC4. |
| Expression host |
Escherichia Coli. |
| Synonyms |
mago-nashi homolog B (Drosophila), mago, magoh, MGN2, MAGOHB, MAGOH2. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMAVASDF YLRYYVGHKG KFGHEFLEFE FRPDGKLRYA NNSNYKNDVM IRKEAYVHKS VMEELKRIID DSEITKEDDA LWPPPDRVGR QELEIVIGDE HISFTTSKIG SLIDVNQSKD PEGLRVFYYL VQDLKCLVFS LIGLHFKIKP I. |
