| Formulation |
The protein was lyophilized without additives. |
| Solubility |
It is recommended to reconstitute the lyophilized CXCL4 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity |
Greater than 95.0% as determined by SDS-PAGE. |
| Description |
CXCL4 Variant-1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 77 amino acids and having a molecular mass of 8.7 kDa. The CXCL4 Variant-1 is fused to 6xHis tag at N-Terminus and purified by standard chromatography techniques. |
| Protein Background |
Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair. Oncostatin-A is a member of the CXC chemocinfamily. Human PF4 is used for the proof of heparin-induced thrombocytopenia. Furthermore it is used as an inhibitor in the angiogenesis during tumor therapy. |
| Expression host |
Escherichia Coli. |
| Synonyms |
CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298. |
| Reagent Appearance |
Sterile Filtered white lyophilized powder. |
| Stability |
Human CXCL4 although stable at 25°C 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles. |
| Amino acid sequence |
MHHHHHHEAE EDGDLQCLCV KTTSQVRPRH ITSLEVIKAG PHCPTAQLIA TLKNGRKICL DLQALLYKKI IKEHLES. |
| link |
Dengue protein is purified by proprietary chromatographic technique. |
