| Reconstitution |
1mg/ml in PBS (after reconstitution). |
| Formulation |
The BLOC1S2 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 50mM NaCl, 1mM DTT and 10% glycerol. |
| Purity |
Greater than 85% as determined by SDS-PAGE. |
| Description |
BLOC1S2 Human Recombinant produced in E. coli is a single polypeptide chain containing 166 amino acids (1-142) and having a molecular mass of 18.5kDa.BLOC1S2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
BLOC1S2 is a member of the BLOC1S2 family. Biogenesis of lysosome-related organelles complex 1 or BLOC-1 is a universally expressed multisubunit protein complex which is essential for regular biogenesis of specialized organelles of the endosomal-lysosomal system, like melanosomes and platelet dense granules. BLOC1S2 takes part in cell proliferation. |
| Expression host |
E.coli. |
| Synonyms |
Biogenesis of lysosomal organelles complex-1 subunit 2, BLOS2, BLOC-1 subunit 2, Centrosome-associated protein, centrosome protein oncogene, centrosomal 10 kDa protein, RP11-316M21.4, CEAP, FLJ30135, MGC10120. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSHMAAAAE GVLATRSDEP ARDDAAVETA EEAKEPAEAD ITELCRDMFS KMATYLTGEL TATSEDYKLL ENMNKLTSLK YLEMKDIAIN ISRNLKDLNQ KYAGLQPYLD QINVIEEQVA ALEQAAYKLD AYSKKLEAKY KKLEKR |
| Antibody Properties |
Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Protein content |
By Western blot 1:2,000 dilution will yield a strong band in WB. |
| Application And Suggested Dilutions |
This antibody was produced in BALB/c miceUsageProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
