More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| coactivator-associated arginine methyltransferase 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human PRMT4(267-284aa HLAPFTDEQLYMEQFTKA), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CARM1 |
Protein | Histone-arginine methyltransferase CARM1 |
Uniprot ID | Q86X55 |
Function | Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg- 2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA- stabilizing properties and the half-life of their target mRNAs. |
Tissue Specificity | Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia. |
Sub-cellular localization | Nucleus. Cytoplasm. Note: Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane. |
Sequence Similarities | Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family. |
Aliases | carm1 antibody|Coactivator associated arginine methyltransferase 1 antibody|Coactivator-associated arginine methyltransferase 1 antibody|Histone arginine methyltransferase CARM 1 antibody|Histone arginine methyltransferase CARM1 antibody|Histone-arginine methyltransferase CARM1 antibody|PRMT 4 antibody|PRMT4 antibody|Protein arginine methyltransferase antibody|Protein arginine N methyltransferase 4 antibody|Protein arginine N-methyltransferase 4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PRMT4 antibody, ASA-B1567, All Western blotting
All lanes: Anti-CARM1(ASA-B1567) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 40ug
Lane 2: Human Placenta Tissue Lysate at 40ug
Lane 3: Rat Kidney Tissue Lysate at 40ug
Lane 4: NIH Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: HL60 Whole Cell Lysate at 40ug
Lane 7: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
All lanes: Anti-CARM1(ASA-B1567) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 40ug
Lane 2: Human Placenta Tissue Lysate at 40ug
Lane 3: Rat Kidney Tissue Lysate at 40ug
Lane 4: NIH Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: HL60 Whole Cell Lysate at 40ug
Lane 7: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
