NMDAR2B  Antibody View larger

NMDAR2B Antibody

ASA-B1391

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

glutamate receptor, ionotropic, N-methyl D-aspartate 2B Polyclonal IgG Rabbit Human, Mouse, Rat WB Immunogen affinity purified.

Immunogen

E.coli-derived human NMDAR2B recombinant protein (Position: N1076-D1332). Human NMDAR2B shares 97.7% and 97.3% amino acid (aa) sequence identity with mouse and rat NMDAR2B, respectively.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

GRIN2B

Protein

Glutamate receptor ionotropic, NMDA 2B

Uniprot ID

Q13224

Function

NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity).

Tissue Specificity

Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.

Sub-cellular localization

Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.

Sequence Similarities

Aliases

AW490526 antibody|Glutamate [NMDA] receptor subunit epsilon 2 antibody| Glutamate [NMDA] receptor subunit epsilon-2 antibody|Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody|Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody|Glutamate receptor ionotropic NMDA2B antibody|Glutamate receptor subunit epsilon 2 antibody|Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody|GRIN 2B antibody|GRIN2B antibody|hNR 3 antibody|hNR3 antibody|MGC142178 antibody|MGC142180 antibody|N methyl D asparate receptor channel subunit epsilon 2 antibody|N METHYL D ASPARTATE RECEPTOR CHANNEL SUBUNIT EPSILON 2 antibody|N methyl D aspartate receptor subtype 2B antibody|N methyl D aspartate receptor subunit 2B antibody|N methyl D aspartate receptor subunit 3 antibody|N-methyl D-aspartate receptor subtype 2B antibody|N-methyl-D-aspartate receptor subunit 3 antibody|NMDA NR2B antibody|NMDA R2B antibody|Nmdar2b antibody|NMDE2 antibody|NMDE2_HUMAN antibody|NME2 antibody|NR2B antibody|NR3 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Mouse, Rat HumanAssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- NMDAR2B antibody, ASA-B1391, Western blotting
All lanes: Anti NMDAR2B (ASA-B1391) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 166KD
Observed bind size: 220KD

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