More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| dopamine receptor D5 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse Dopamine Receptor D5(388-407aa LISYNQDTVFHREIAAAYVH), different from the related rat sequence by two amino acids, and from the related human sequence by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DRD5 |
Protein | D(1B) dopamine receptor |
Uniprot ID | Q8BLD9 |
Function | Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. |
Sequence Similarities | |
Aliases | D(1B) dopamine receptor antibody|D(5) dopamine receptor antibody|D1 beta dopamine receptor antibody|D1B dopamine receptor antibody|D1beta dopamine receptor antibody|D5 dopamine receptor antibody|DBDR antibody|Dopamine D5 receptor antibody|Dopamine receptor D1B antibody|DRD 1B antibody|DRD 5 antibody|DRD1B antibody|DRD1L2 antibody|DRD5 antibody|DRD5_HUMAN antibody|MGC10601 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Dopamine Receptor D5 antibody, ASA-B0606, Western blotting
All lanes: Anti Dopamine Receptor D5 (ASA-B0606) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Rat Brain Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 58KD
All lanes: Anti Dopamine Receptor D5 (ASA-B0606) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Rat Brain Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 58KD
