More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| superoxide dismutase 3, extracellular | Polyclonal | IgG | Rabbit | Human | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Superoxide Dismutase 3(41-56aa KVTEIWQEVMQRRDDD). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SOD3 |
Protein | Extracellular superoxide dismutase[Cu-Zn] |
Uniprot ID | P08294 |
Function | Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. |
Tissue Specificity | Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid. |
Sub-cellular localization | Secreted, extracellular space. Note: 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium. |
Sequence Similarities | Belongs to the Cu-Zn superoxide dismutase family. |
Aliases | EC SOD antibody|EC-SOD antibody|Extracellular superoxide dismutase[Cu Zn] antibody|Extracellular superoxide dismutase[Cu-Zn] antibody|Extracellular superoxide dismutase antibody|Extracellular superoxide dismutase precursor antibody|MGC20077 antibody|SOD 3 antibody|SOD3 antibody|SODE_HUMAN antibody|Superoxide dismutase 3 extracellular antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Superoxide Dismutase 3 antibody, ASA-B1812, Western blotting
Lane 1: Human Placenta Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: MM231 Cell Lysate
Lane 4: MCF-7 Cell Lysate
Lane 1: Human Placenta Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: MM231 Cell Lysate
Lane 4: MCF-7 Cell Lysate
Anti-Superoxide Dismutase 3 antibody, ASA-B1812, IHC(F)
IHC(F): Human Placenta Tissue
IHC(F): Human Placenta Tissue
